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Primer
Primer
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Optimum Primer For Every PCR & qPCR Assay
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![A Python script to design primers for overlap extension PCR to ligate two DNA fragments [PeerJ]](https://dfzljdn9uc3pi.cloudfront.net/2022/14283/1/fig-1-2x.jpg "A Python script to design primers for overlap extension PCR to ligate two DNA fragments [PeerJ]")